蛋白标记：纳米材料的实际应用-百灵威

在当今生物医学研究领域，蛋白标记技术对于深入了解蛋白质的功能、分布以及相互作用起着至关重要的作用。而纳米材料凭借其独特的物理化学性质，为蛋白标记带来了革命性的突破，极大地推动了相关研究的发展。接下来，让我们一同探索几种关键纳米材料在蛋白标记中的神奇功能。

光学信号标记

在光学信号标记领域，金纳米棒、金纳米颗粒、银纳米颗粒与量子点各有所长：金纳米棒能调峰探癌细胞；金纳米颗粒可变色助免疫标记；银纳米颗粒检测灵敏度高；量子点能多色标记追踪蛋白，精准区分信号。

百灵威可以提供的用于光学信号标记的纳米材料有：

品名	CAS	货号
Gold, 99%, 0.1 mg/mL dispersion in H2O, diam.: 40±3 nm, length: 80±10 nm, λ: 660±5 nm 金	7440-57-5	129871
Gold nanorods, particle size:14nm×50nm (diam. × L ),80 μg/mL in H2O 金纳米棒	7440-57-5	928224
Oil soluble gold nanorods, 0.1 mg/mL in in xylenes, surface group: arenes, λ=1220±15nm 油溶性金纳米棒	/	9229303
Oil soluble gold nanorods, 0.1 mg/mL in in xylenes, surface group: arenes, λ=1260±15nm 油溶性金纳米棒	/	9229304
Cadmium selenide StremDots? quantum dot (CdSe core) kit, 50umol/L in hexanes, 525-625nm peak emissions 硒化镉	1306-24-7	96-0800 危化品
Cadmium selenide StremDots? quantum dot (CdSe core), 50umol/L in hexanes, 525nm peak emission 硒化镉	1306-24-7	48-1011 危化品
Cadmium sulfide/Zinc sulfide core/shell quantum dots with Amine in water Emission peak: 400 nm FWHM <35nm QY>50% 硫化镉/硫化锌核/壳量子点	/	48-1658
Amino functional graphene quantum dots, 1 mg/mL dispersion in water, fluorescence: λem 429 nm 氨基化石墨烯量子点	1034343-98-0	959913
Carboxyl functional graphene quantum dots, 1 mg/mL dispersion in water, fluorescence: λem 487 nm 羧基化石墨烯量子点	1034343-98-0	909651
Water soluble silver nanoparticles, 0.1 mg/mL in ultrapure water, d=100±5nm, surface group: citrate, λ=490±10nm, OD:1±0.2 水溶性银纳米颗粒	/	9229065
Water soluble silver nanoparticles, 0.1 mg/mL in ultrapure water, d=30±5nm, surface group: citrate, λ=410±5nm, OD:1±0.2 水溶性银纳米颗粒	/	9229058
Oil soluble silver nanoparticles, 0.1 mg/mL in in xylenes, d=10±2nm, surface group: arene 油溶性银纳米颗粒	/	9229067
Oil soluble silver nanoparticles, 0.1 mg/mL in in xylenes, d=20±3nm, surface group: arene 油溶性银纳米颗粒	/	9229069
Au@SiO2 nanoparticles, 0.05 mg/mL in ultrapure water, Au=15nm, thickness of SiO2: 25nm, surface group: SiO2 Au@SiO2纳米颗粒	/	9229037
Au@SiO2 nanoparticles, 0.05 mg/mL in ultrapure water, Au=40nm, thickness of SiO2: 25nm, surface group: SiO2 Au@SiO2纳米颗粒	/	9229046
Spherical gold nanoparticles modified with streptavidin, 0.05 mg/mL in ultrapure water, alternative diameter: 5-200nm optional, surface group: SA 链霉亲和素修饰的球形金纳米颗粒	/	9229267
Oil soluble spherical gold nanoparticles, 0.05 mg/mL in xylenes, d=100±5nm, surface group: arenes 油溶性球形金纳米颗粒	/	9229233
Oil soluble spherical gold nanoparticles, 0.05 mg/mL in xylenes, d=10±3nm, surface group: arenes 油溶性球形金纳米颗粒	/	9229226
Water soluble spherical gold nanoparticles, 0.05 mg/mL in ultrapure water, d=10±3nm, surface group: citrate, λ=519±2nm, OD: 0.8±0.1 水溶性球形金纳米颗粒	7440-57-5	919290
Water soluble spherical gold nanoparticles, 0.05 mg/mL in ultrapure water, d=90±5nm, surface group: citrate, λ=560±5nm, OD: 1.0±0.2 水溶性球形金纳米颗粒	/	9229218
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=100±5nm, surface group: —PEG—COOH PEG化球形金纳米颗粒	/	9229261
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=100±5nm, surface group: —PEG—NH2 PEG化球形金纳米颗粒	/	9229247
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=10±3nm, surface group: —PEG—COOH PEG化球形金纳米颗粒	/	9229254

信号增强

在信号增强上，金纳米颗粒与量子点作用关键。金纳米颗粒靠表面等离子体共振，免疫金标记时能以颜色显蛋白结合态，作 SERS 基底可增强拉曼信号；量子点以高量子产率发强荧光、峰窄对称，多色标记追踪蛋白时信号清晰强烈，助精准检测。

百灵威可以提供的有信号增强作用的纳米材料有：

品名	CAS	货号
Au@SiO2 nanoparticles, 0.05 mg/mL in ultrapure water, Au=15nm, thickness of SiO2: 25nm, surface group: SiO2 Au@SiO2纳米颗粒	/	9229037
Au@SiO2 nanoparticles, 0.05 mg/mL in ultrapure water, Au=40nm, thickness of SiO2: 25nm, surface group: SiO2 Au@SiO2纳米颗粒	/	9229046
Spherical gold nanoparticles modified with streptavidin, 0.05 mg/mL in ultrapure water, alternative diameter: 5-200nm optional, surface group: SA 链霉亲和素修饰的球形金纳米颗粒	/	9229267
Oil soluble spherical gold nanoparticles, 0.05 mg/mL in xylenes, d=100±5nm, surface group: arenes 油溶性球形金纳米颗粒	/	9229233
Oil soluble spherical gold nanoparticles, 0.05 mg/mL in xylenes, d=10±3nm, surface group: arenes 油溶性球形金纳米颗粒	/	9229226
Water soluble spherical gold nanoparticles, 0.05 mg/mL in ultrapure water, d=10±3nm, surface group: citrate, λ=519±2nm, OD: 0.8±0.1 水溶性球形金纳米颗粒	7440-57-5	919290
Water soluble spherical gold nanoparticles, 0.05 mg/mL in ultrapure water, d=90±5nm, surface group: citrate, λ=560±5nm, OD: 1.0±0.2 水溶性球形金纳米颗粒	/	9229218
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=100±5nm, surface group: —PEG—COOH PEG化球形金纳米颗粒	/	9229261
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=100±5nm, surface group: —PEG—NH2 PEG化球形金纳米颗粒	/	9229247
Spherical gold nanoparticles modified with PEG, 0.05 mg/mL in ultrapure water, d=10±3nm, surface group: —PEG—COOH PEG化球形金纳米颗粒	/	9229254
Cadmium selenide StremDots? quantum dot (CdSe core) kit, 50umol/L in hexanes, 525-625nm peak emissions 硒化镉	1306-24-7	96-0800 危化品
Cadmium selenide StremDots? quantum dot (CdSe core), 50umol/L in hexanes, 525nm peak emission 硒化镉	1306-24-7	48-1011 危化品
Cadmium sulfide/Zinc sulfide core/shell quantum dots with Amine in water Emission peak: 400 nm FWHM <35nm QY>50% 硫化镉/硫化锌核/壳量子点	/	48-1658
Amino functional graphene quantum dots, 1 mg/mL dispersion in water, fluorescence: λem 429 nm 氨基化石墨烯量子点	1034343-98-0	959913
Carboxyl functional graphene quantum dots, 1 mg/mL dispersion in water, fluorescence: λem 487 nm 羧基化石墨烯量子点	1034343-98-0	909651

活性调节

氧化锌纳米颗粒可与蛋白互作影响活性，吸附蛋白表面改变微环境来调节，如结合酶蛋白影响其活性中心构象、催化效率，这为研究蛋白功能与开发生物传感器提供思路，调节机制因蛋白和条件而异。

百灵威可以提供的具有活性调节功能的纳米材料有：

品名	CAS	货号
Zinc oxide, 99.99%, trace metals basis 氧化锌	1314-13-2	955126
Zinc oxide nanopowder 氧化锌	1314-13-2	30-1405

蛋白标记：纳米材料的实际应用

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