CRISPR-Cas9等基因编辑技术的爆发式发展,将 细胞培养与转染技术推向了前所未有的重要位置。基因编辑并非在真空中进行,其高效的筛选、验证和应用都离不开体外培养的细胞。细胞培养为基因编辑提供了纯净、可增殖的“加工厂”,而转染技术则是将CRISPR“基因剪刀”组件精准递送到细胞核内的关键运输工具。无论是质粒、RNA还是核糖核蛋白复合体的递送,转染的效率与细胞毒性直接决定了基因编辑的成功率与实验周期。细胞培养与转染技术为在细胞水平上对基因组进行定点敲除、敲入或单碱基替换成为常规操作奠定了基石,从而能够快速构建疾病模型、筛选功能基因和验证药物靶点。
百灵威提供细胞培养和转染相关的试剂及仪器耗材。
产品优势:质控严格,货源稳定。
| 品名 | CAS | 货号 |
|---|---|---|
| RPMI 1640 RPMI 1640细胞培养基 | / | 9328571 |
| MEM, Reduced Serum Medium, no Phenol Red MEM, 减血清培养基, 不含酚红 | / | 9328569 |
| DMEM, high glucose, pyruvate DMEM, 高糖, 含丙酮酸盐 | / | 923273 |
| Ham's F-12K(Kaighn's) Nutrient Mix with Phenol Red and L-glutamine Ham's F-12K(Kaighn's) 营养培养基 | / | 9328561 |
| 品名 | CAS | 货号 |
|---|---|---|
| Superior fetal bovine serum 优级胎牛血清 | / | MN012103促 |
| Special grade fetal bovine serum 特级胎牛血清 | / | MN112103促 |
| Fetal bovine serum 胎牛血清 | / | 173427 |
| Superior fetal bovine serum 精制胎牛血清 | / | 9410422 |
| Superior fetal bovine serum, heat inactivated 精制胎牛血清 | / | 9450540 |
| 品名 | CAS | 货号 |
|---|---|---|
| β-Nicotinamide adenine dinucleotide disodium salt, 98%, reduced β-烟酰胺腺嘌呤二核苷酸二钠盐 | 606-68-8 | 302449 |
| L-Ascorbic acid, 99% 抗坏血酸 | 50-81-7 | 231406 |
| Calcium pantothenate, 98% 泛酸钙 | 137-08-6 | 183074 |
| Retinoic acid, 98.5%, a natural agonist of RAR nuclear receptors, with IC50 of 14 nM for RARα/β/γ, Kd of 17 nM for PPARβ/δ 维甲酸 | 302-79-4 | 961027 |
| Nicotinic acid, 99.5% 烟酸 | 59-67-6 | 978096 |
| (+)-Sodium L-ascorbate, 99% (+)-L-抗坏血酸钠 | 134-03-2 | 171771 |
| D-Biotin, 99% D-生物素 | 58-85-5 | 322564 |
| (2-Hydroxypropyl)-β-cyclodextrin, 97%, reagent grade (2-羟丙基)-β-环糊精 | 128446-35-5 | 957471 |
| Riboflavin, 98% 维生素B2 | 83-88-5 | 469220 |
| 品名 | CAS | 货号 |
|---|---|---|
| RFect小核酸转染试剂,0.1ml RFect小核酸转染试剂,0.1ml | / | 11031 |
| Rfect V2 siRNA转染试剂,0.5ml Rfect V2 siRNA转染试剂,0.5ml | / | 11041 |
| RFect mRNA转染试剂盒,0.1ml RFect mRNA转染试剂盒,0.1ml | / | 21057 |
| RFect质粒DNA转染试剂盒,0.1ml RFect质粒DNA转染试剂盒,0.1ml | / | 21040 |
| RFectPM原代细胞质粒DNA转染试剂盒,0.1ml RFectPM原代细胞质粒DNA转染试剂盒,0.1ml | / | 21041 |
| RFect293细胞DNA转染试剂盒,0.1ml RFect293细胞DNA转染试剂盒,0.1ml | / | 21047 |
| RFect Prime核酸转染试剂盒,0.1ml RFect Prime核酸转染试剂盒,0.1ml | / | 21043 |
| 品名 | CAS | 货号 |
|---|---|---|
| Single-channel mechanical pipettor, Volume range: 100 - 1000 μL, Increment: 5.0 μL 单道手动可调移液器 | / | 1122427 |
| Pipette tips, 1000 μL, blue, 1000 tips/pack 移液器吸头 | / | 672890 |
| 4.5mL External thread cryogenic tube, Tube cover integrated, bag sterilization, transparent,50 tips/pack, 300 tips/box, 1200 tips/case 4.5mL外旋冻存管 | / | 9451890 |
| 2mL Radiation-resistant centrifuge tube, Non-sterile bagged, transparent, 50 tips/pack, 5 packs/box, 10 boxes/case 2mL耐辐照离心管 | / | 9451937 |
| 24-well LACsurface flat-bottom cell culture plate, Sterilized box,1tips/box, 50boxes/case 24孔LACsurface平底细胞培养板 | / | 9452027 |