细菌质粒是重组DNA技术中常用的基因运载工具。碱裂解法是十分常见的少量制备质粒DNA的方法。^[1]

百灵威提供碱裂解法提取质粒DNA的相关试剂、移液器和耗材。

产品列表

分类	品名	货号
溶液Ⅰ（GTE溶液）
	Tris(hydroxymethyl)aminomethane, 99.5%, extra pure 三(羟甲基)氨基甲烷	226162
	Ethylenediaminetetraacetic acid, 99%, reagent grade 乙二胺四乙酸	932641
	D-(+)-Glucose, 99%, for bio D-(+)-葡萄糖	936778
溶液Ⅱ
	Sodium hydroxide, 96%, for analysis, flaky 氢氧化钠	904646
	Sodium dodecyl sulfate, 99%, for biochemistry 十二烷基硫酸钠	105893
溶液Ⅲ
	Acetic acid, 99.5%, for analysis 乙酸	991956
	Potassium acetate, 99%, for analysis 乙酸钾	978296
LB培养液
	Yeast extract powder 酵母提取粉	716769
	Sodium chloride, 99.5%, molecular biology grade 氯化钠	908606
RNase A溶液
	Ribonuclease A, from bovine pancreas, ≥50 Kunitz units/mg 核糖核酸酶 A	139924
TE缓冲溶液
	Tris(hydroxymethyl)aminomethane, 99.5%, extra pure 三(羟甲基)氨基甲烷	226162
	Ethylenediaminetetraacetic acid, 99%, reagent grade 乙二胺四乙酸	932641
其他试剂
	Ethanol, 99.7%, reagent grade, anhydrous 乙醇	983081
	Isopropanol, 99.7%, for synthesis 异丙醇	923740
	Ethanol, 99.7%, reagent grade, anhydrous 乙醇	983081
	Kanamycin sulfate, an aminoglycoside bacteriocidal antibiotic which acts by binding to the bacterial 30S ribosomes 硫酸卡那霉素	357211
小型仪器
	Single-channel mechanical pipettor, Volume range: 0.5 - 10 μL, Increment: 0.1 μL 单道手动可调移液器	1122421
	Single-channel mechanical pipettor, Volume range: 20 - 200 μL, Increment: 1.0 μL 单道手动可调移液器	954106
	Single-channel mechanical pipettor, Volume range: 100 - 1000 μL, Increment: 5.0 μL 单道手动可调移液器	1122427
基础耗材
	Centrifuge tubes, 1.5 mL, clear, 500 tubes/pack 离心管	915664
	Pipette tips, 10 μL, clear, 1000 tips/pack 移液器吸头	920067
	Pipette tips, 200 μL, yellow, 1000 tips/pack 移液器吸头	970956
	Pipette tips, 1000 μL, blue, 1000 tips/pack 移液器吸头	672890

实验步骤

可点击右上角箭头下载

参考文献

1. 刘长霞, 罗施中. 分子生物学实验技术[M]. 北京:化学工业出版社,2018.

相关阅读

常用生化试剂一览
生化常用培养基及相关试剂的配制
常用生化试剂配制